RhoB regulates uPAR signalling

RhoB regulates uPAR signalling.

Urokinase-type plasminogen activator (uPA) and its receptor, uPAR, play important roles in promoting cancer cell adhesion, migration and invasion. Rho GTPases are key coordinators of these processes; the Rho GTPase Rac1 has previously been implicated in uPA- and/or uPAR-induced migratory or morphological cell responses. We used RNAi to deplete 12 different Rho GTPases to screen for effects on u...

Two signalling hands for uPAR.

doi:10.1160/TH13-01-0001 Thromb Haemost 2013; 109: 179 T&H Insight on ▶Montuori et al. Thromb Haemost 2013; 109: 309-318. Transmembrane proteins usually relay signals to the cytoplasm and nucleus via their intracellular domains. Nonetheless, this paradigm is challenged by glycosylphosphatidylinositol (GPI)-anchored proteins. Although completely extracellular, these proteins are linked to the li...

RhoA regulates RhoB through a RhoGDIα-dependent mechanism

The Small GTPase RhoB Regulates TNFα Signaling in Endothelial Cells

The inflammatory response of endothelial cells triggered by cytokines such as TNFα and IL1β plays a pivotal role in innate immunity. Upon pro-inflammatory cytokine stimulation, endothelial cells produce chemokines and cytokines that attract and activate leukocytes, and express high levels of leukocyte adhesion molecules. This process is mediated by intracellular signaling cascades triggered by ...

RhoB regulates cell migration through altered focal adhesion dynamics

The Rho GTPase RhoB has been shown to affect cell migration, but how it does this is not clear. Here we show that cells depleted of RhoB by RNAi are rounded and have defects in Rac-mediated spreading and lamellipodium extension, although they have active membrane ruffling around the periphery. Depletion of the exchange factor GEF-H1 induces a similar phenotype. RhoB-depleted cells migrate faste...